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- Other Names
- BMP2A, BMP-2A, BMP-2, Bone Morphogenetic Protein 2A
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| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 767314 | 25 µg | £589 | ||||
| 767316 | 100 µg | £1505 | ||||
Select size of product is eligible for a 40% discount! Promotion valid until September 30, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
Description
Bone morphogenetic protein 2 (BMP-2) belongs to the TGF-β superfamily. Like other members of BMP family, BMP-2 is synthesized as an inactive propeptide precursor which dimerizes and then, it is further processed into mature form by proprotein convertases (PCs). Some evidence indicated that PC5/6A and Factor VII-activating protease (FSAP) are involved in maturation of BMP-2. Mature BMP-2 is a 26 kD protein composed of 114 amino acids, forming three intramolecular and one intermolecular disulfide bond. BMP-2 forms hom*odimer or heterodimer with other BMP proteins, including BMP-4, BMP-6 and BMP-7. BMP-2 signal through heterodimeric serine/threonine kinase receptors composed of a type I (BMPR1a/ALK3, BMPR1b/ALK6, ActRIa/ALK2) and a type II (BMPR2, ACVR2a/ActRIIA, ACVR2b/ActRIIB). BMP-2 binds to the type I receptor with high affinity, in turn recruiting the type II receptor. BMP-2 stimulation initiates receptor shutdown, leading to receptor clustering and activation of the downstream signaling. BMP-2 signals via canonical Smad or other downstream kinase, such as p38 and JNK in a context-dependent manner. BMP-2 is involved in several processes, including cartilage and bone formation, differentiation, and embryogenesis. BMP-2 induces osteogenic differentiation in human mesenchymal stem cells and myogenic cells. BMP-2 induces cartilage repair and remodeling by stimulating chondrocyte proliferation and expression of matrix proteins. BMP-2/BMP-7 heterodimer is more potent in the induction of bone formation in vivo than BMP-2 hom*odimer. BMP-2-deficiency leads to embryonic lethality with abnormal cardiac development, malformation of the amnion/chorion, severe chondrodysplasia, and defects in myocardial patterning, suggesting that BMP-2 mediates organ morphogenesis. Noggin is an antagonist that can reverse BMP-2-mediated effect. Noggin exppression is induced by BMP-2 in osteoblasts as a negative feedback loop. In addition, BMP-2 stimulates epithelial to mesenchymal cell transformation through TGF-β type III receptor.
Product Details
Quality Statement
BioLegend Cell-Vive™ GMP Recombinant proteins are manufactured and tested in accordance with USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and Ph. Eur. Chapter 5.2.12 in a dedicated GMP facility compliant with ISO 13485:2016. Specifications and processes include:
- Low endotoxin level (≤ 0.1 EU/μg)
- Purity (≥ 95% or higher)
- Bioburden testing
- Mycoplasma testing
- Batch-to-batch consistency
- Vendor qualification
- Raw material traceability and documentation
- Documented procedures and employee training
- Equipment maintenance and monitoring records
- Lot-specific certificates of analysis
- Quality audits per ISO 13485:2016
- QA review of released products
Product Details
- Source
- Human BMP-2, amino acid (Ala284-Arg396) (Accession: # P12643) was expressed in E.coli.
- Molecular Mass
- The 113 amino acid recombinant protein has a predicted molecular mass of approximately 12.8 kD. The DTT-reduced and non-reduced protein migrates at approximately 13 and 26 kD respectively by SDS-PAGE. The predicted N-terminal amino acid is Ala.
- N-terminal
Sequence Analysis - Ser-Ile-Thr-Gly-Ile-Ser-Pro-Ile-Thr-Glu
- Purity
- ⥠95%, as determined by Coomassie stained SDS-PAGE.
- Formulation
- 0.1 µm filtered protein solution is in 4 mM HCl
- Endotoxin Level
- Less than or equal to 0.1 EU per μg protein as determined by the LAL method.
- Residual Host Cell Protein Content
- ⤠0.500 ng/μg by ELISA
- Concentration
- 25 µg and 100 µg sizes are bottle at 0.5 mg/mL
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% endotoxin-free BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week or stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
- Activity
- Human BMP-2 induces alkaline phosphatase (ALP) production in the mouse chrondrogenic cell line ATDC5 in a dose dependent manner. The ED50 for this effect is 40 â 200 ng/mL.
- Application
- Bioassay
- Application Notes
BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at [emailprotected].
- Disclaimer
BioLegend Cell-Vive™ GMP Recombinant proteins are for research use only. Suitable for ex vivo cell processing. Not for injection or diagnostic or therapeutic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.
Antigen Details
- Structure
- Disulfide-linked hom*odimer
- Distribution
-
Abundant in lung, spleen and colon in human, low expression but detectable in heart, kidney, brain, liver, skeletal muscle, pancreases, placenta, prostate, ovary, and small intestine
- Function
- BMP-2 is involved in osteogenesis, cartilage repair, and organogenesis
- Interaction
- Chondroblast, osteoblast, proprotein convertases, BMP family members
- Ligand/Receptor
- BMP receptor type IA (BMPR1a/ALK3), type IB (BMPR1b/ALK6), Activin receptor type IA (ACVR1a/ActRIa/ALK2) and BMP receptor type II (BMPR2), Activin receptors type IIA (ACVR2a/ActRIIA), type IIB (ACVR2b/ActRIIB)
- Bioactivity
- BMP-2 induces alkaline phosphatase in ATDC5 mouse chondrogenic cells.
- Cell Type
- Embryonic Stem Cells
- Biology Area
- Angiogenesis, Cell Biology, Neuroscience, Stem Cells, Synaptic Biology
- Molecular Family
- Cytokines/Chemokines, Growth Factors
- Antigen References
-
- Bragdon B,et al. 2011.Cell Signal23: 609.
- Israel DI,et al. 1992.Growth Factor.7: 139.
- Lee SN,et al. 2015.Am. J. Respir. Cell Mol. Biol.52: 749.
- Roedel EK,et al. 2013.J. Biol. Chem.288, 7193.
- Nohe A,et al. 2002.J. Biol. Chem.277: 5330.
- Zhou AJ,et al. 2012.Growth Factors30: 267.
- Miyazono K,et al. 2010.J. Biochem.147: 35.
- Nohe Aet al. 2004.J. Cell Sci.118: 643.
- Hay E,et al. 2001.J. Biol. Chem.276: 29028.
- Wozney JM,et al. 1988.Science242: 1528.
- Ryoo HM,et al. 2006.Gene366: 51.
- Lavery K,et al. 2008.J. Biol. Chem.283: 20948.
- Israel DI,et al. 1996.Growth Factors13: 291.
- Mundy G,et al. 1999.Science286: 1946.
- De Luca Fet al. 2001.Endocrinology142: 430.
- Blaney Davidson EN,et al. 2007.Arthritis Res. Ther.9: R102.
- Zhang H, Bradley A. 1996.Development122: 2977.
- Pera MF,et al. 2004.J. Cell Sci.117: 1269.
- Wang RN,et al. 2014.Genes Dis.87-105.
- Kirkbride KC,et al. 2008.J. Biol. Chem.283: 7628.
- Gene ID
- 650 View all products for this Gene ID
- UniProt
- View information about BMP-2 on UniProt.org
Documentation
- Technical Data Sheet (pdf)
- Certificate of Analysis
- Safety Data Sheet
Reviews
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| Description | Clone | Applications |
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Pathways
- Stem Cells and Development
Pages
- Quality Control
- Multicolor Staining Guide
- Recombinant Proteins
- Fluorescence Spectra Analyzer
- Fluorophore Brightness Index
- Custom Panel Construction
- Fluorophore Equivalency Tool
Related FAQs
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Certificate of Analysis
For Research Use Only. Suitable for ex vivo cell processing. Not for injection or diagnostic or therapeutic use.
This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for userâs intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.
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